FastPrep-24™ 5G Bead beating Grinder and Lysis System from MP Biomedicals

Product Specs
ItemFastPrep-24™ 5G Bead beating Grinder and Lysis System
CompanyMP Biomedicals
Price Get Pricing Get Quote for this Product
Catalog Number116005500
QuantityEA
TypeBenchtop
ApplicationsLysis of yeast, fungi, and bacteria, soft tissues, cells, Isolation of PCR-Ready Genomic DNA from fecal or soil samples. Isolation of PCR-Ready Genomic DNA from fungi and bacteria samples. DNA & RNA Purification. Breaks hard/brittle samples. Isolate DNA from virtually any source. Lysis of highly contagious samples. Grinding of teeth, bones, hair and pulverization of building materials.
Capacity~200 µL to 50 mL; up to ~25 g

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MP Biomedicals

Thüringer Str. 15

Eschwege 37269

Germany

Phone: +33 3 88 67 54 25

Email: info.europe@mpbio.com

Company Profile

Website: http://www.mpbio.com

(1) Exploring extracellular matrix and prostaglandin pathway alterations across varying resection margin distances of right-sided colonic adenocarcinomaBMC CancerDecember 7, 2023Tharathorn Suwatthanarak, Pariyada Tanjak, Thanawat Suwatthanarak, Onchira Acharayothin, Kullanist Thanormjit, Amphun Chaiboonchoe, Thikhamporn Tawantanakorn, Chainarong Phalanusitthepha, Atthaphorn Trakarnsanga, Asada Methasate, et al.1. RNA extraction and qualification Fresh specimens weighing 20–30 mg were homogenized using the FastPrep-24™ 5G bead beating grinder and lysis system (MP Biomedicals, CA, USA). The resulting lysate was then subjected to a standard total RNA isolation

(2) Time-restricted feeding attenuates hypercholesterolaemia and atherosclerosis development during circadian disturbance in APOE∗3-Leiden.CETP miceeBioMedicineJuly 1, 2023Wietse In Het Panhuis, Milena Schönke, Melanie Modder, Hannah E. Tom, Reshma A. Lalai, Amanda C.M. Pronk, Trea C.M. Streefland, Linda W.M. van Kerkhof, Martijn E.T. Dollé, Marie A.C. Depuydt, et al.homogenization using TriPure RNA Isolation Reagent (11667165001, Sigma–Aldrich, Saint Louis, USA) and a FastPrep-24™ 5G bead beating grinder and lysis system (4.0 m/s for 10 s; MP Biomedicals™, Santa Ana, California, USA). cDNA was synthesized from 1 μg RNA

(3) Granulins rescue inflammation, lysosome dysfunction, and neuropathology in a mouse model of progranulin deficiencybioRxivApril 19, 2023Jessica Root, Anarmaa Mendsaikhan, Srijita Nandy, Georgia Taylor, Minzheng Wang, Ludmilla Troiano Troiano Araujo, Paola Merino, Danny Ryu, Christopher Holler, Bonne M. Thompson, et al.spiked with internal standards (see LCMS methods below) was added to each sample and homogenized with FastPrep-24™ 5G bead beating grinder and lysis system using Lysing Matrix D tubes with CoolPrep™ adapter (MP Biomedicals) for 40 seconds at a speed of 6 m/s.

(4) Aging attenuates diurnal lipid uptake by brown adipose tissueAging (Albany NY)October 15, 2022Wietse In het Panhuis, Milena SchÃ¶nke, Ricky Siebeler, Salwa Afkir, Rianne Baelde, Amanda C.M. Pronk, Trea C.M. Streefland, Hetty C.M. Sips, Reshma A. Lalai, Patrick C.N. Rensen, et al.protease and phosphatase inhibitors (A32959, Thermo Fisher Scientific, Waltham, MA, USA), homogenized by a FastPrep-24™ 5G bead beating grinder and lysis system (4.0 m/s for 10 sec; MP Biomedicals™, Santa Ana, CA, USA) and centrifuged repeatedly (16.2 g for 5 min

(5) KLF4 is a therapeutically tractable brake on fibroblast activation which promotes resolution of pulmonary fibrosisJCI InsightAugust 22, 2022Loka Raghu Kumar Penke, Jennifer M. Speth, Steven K. Huang, Sean M. Fortier, Jared Baas, Marc Peters-Goldentissues harvested from saline- or bleomycin-treated mice, samples were homogenized in TRIzol using the FastPrep-24 5G bead beating grinder and lysis system (MP Biomedicals). Total RNA was extracted using the RNeasy Mini Kit (QIAGEN) and quantified using a

(6) The Host Response to Influenza A Virus Interferes with SARS-CoV-2 Replication during CoinfectionJournal of VirologyAugust 10, 2022Kohei Oishi, Shu Horiuchi, Judith M. Minkoff, Benjamin R. tenOeverhistological analysis or homogenized in lysing matrix A homogenization tubes (MP Biomedicals) in a FastPrep-24 5G bead-beating grinder and lysis system (MP Biomedicals). Lungs for plaque assay were homogenized with an amount of PBS according to their weight.

(7) A diminished immune response underlies age-related SARS-CoV-2 pathologiesCell reportsJune 28, 2022Oishi K, Horiuchi S, Frere J, Schwartz RE, tenOever BR.isolation, lung tissues were homogenized in Lysing Matrix A homogenization tubes (MP Biomedicals) in a FastPrep-24 5G bead beating grinder and lysis system (MP Biomedicals). Lung lobes for plaque assay were homogenized with an amount of PBS according to their

(8) Angiopoietin-like 4 governs diurnal lipoprotein lipase activity in brown adipose tissueMolecular MetabolismJune 1, 2022Robin van Eenige et al.isolated using the nucleospin kit (Macherey–Nagel, Düren, Germany; 740955.50) after homogenization by a FastPrep-24™ 5G bead beating grinder and lysis system (4.0 m s−1, 10 s; MP Biomedicals™, Santa Ana, California, USA) in a mixture of 400 μL RA1 (Macherey–Nagel,

(9) The first fungi: mode of delivery determines early life fungal colonization in the intestine of preterm infantsMicrobiome Research ReportsJanuary 28, 2022Jannie G. E. Henderickx, Heleen de Weerd, Liesbeth J. Groot Jebbink, Diny van Zoeren-Grobben, Marieke A. C. Hemels, Richard A. van Lingen, Jan Knol, Clara Belzer03335208001, Roche Diagnostics) were added and bead-beaten three times at 5.5 ms for 60 s with 15 s pause (FastPrep-24 5G bead beating grinder and lysis system, MP Biomedicals). Subsequently, samples were incubated for 15 min at 95 °C at 100 rpm after which they

(10) Microbial associations of shallow-water Mediterranean marine cave Solenogastres (Mollusca)PeerJDecember 15, 2021Elena Vortsepneva, Pierre Chevaldonné, Alexandra Klyukina, Elizaveta Naduvaeva, Christiane Todt, Anna Zhadan, Alexander Tzetlin, Ilya KublanovDNeasy PowerLyzer Microbial Kit (Qiagen, Germany) according to the manufacturer’s instructions using FastPrep-24™ 5G bead beating grinder and lysis system (MP Biomedicals, USA). The concentration of isolated DNA was measured using Qubit™ dsDNA HS Assay Kit

(11) Equine grass sickness (a multiple systems neuropathy) is associated with alterations in the gastrointestinal mycobiomeAnimal MicrobiomeDecember 1, 2021Bruce C. McGorum, Zihao Chen, Laura Glendinning, Hyun S. Gweon, Luanne Hunt, Alasdair Ivens, John A. Keen, R. Scott Pirie, Joanne Taylor, Toby Wilkinson, et al.(QIAGEN, Hilden, Germany), following manufacturer’s instructions. A bead beating step was included (FastPrep-24 5G bead beating grinder and lysis system, MP Biomedicals, Eschwege, Germany). The purity of the resulting DNA extract was assessed using NanoDrop

(12) From Mum to Bum: An Observational Study Protocol to Follow Digestion of Human Milk Oligosaccharides and Glycoproteins from Mother to Preterm InfantNutrientsSeptember 28, 2021Jannie G. E. Henderickx, Esther J. d’Haens, Marieke A. C. Hemels, Mariëtte E. Schoorlemmer, Astrid Giezen, Richard A. van Lingen, Jan Knol, Clara BelzerCat. No. 03335208001) will be added and bead-beaten three times at 5.5 ms for 60 s with 15 s pause (FastPrep-24™ 5G bead beating grinder and lysis system, MP Biomedicals, Irvine, United States). Subsequently, samples will be incubated for 15 min at 95 °C

(13) Gold nanoparticles affect the cryopreservation efficiency of in vitro-derived shoot tips of bleeding heartPlant Cell, Tissue and Organ Culture (PCTOC)August 1, 2021Dariusz Kulus, Alicja Tymoszukwell as the genotype standard) were analyzed. Total genomic DNA was isolated from fresh tissues using a FastPrep-24 5G bead beating grinder and lysis system (MP Biomedicals Irvine, CA, USA) and Genomic Mini AX Plant Spin kit (A&A Biotechnology, Gdynia, Poland),

(14) Leveraging the antiviral type I interferon system as a first line of defense against SARS-CoV-2 pathogenicityImmunityMarch 1, 2021Daisy A. Hoagland et al.homogenized in Lysing Matrix A homogenization tubes (MP Biomedicals) for 40 s at 6.00 m/s for 2 cycles in a FastPrep-24 5G bead beating grinder and lysis system (MP Biomedicals). All tissues for mRNA-Seq analysis were homogenized directly in 1ml TRIzol (Invitrogen).

(15) GSK3ß inhibitor CHIR 99021 modulates cerebral organoid development through dose-dependent regulation of apoptosis, proliferation, differentiation and migrationPloS oneJanuary 1, 2021Delepine C, Pham VA, Tsang HWS, Sur M.(PhosSTOP, Roche) per respective manufacturer’s protocol. Samples were lysed through shearing force with the FastPrep-24 5G bead beating grinder and lysis system (MP Biomedicals). Overall sample protein levels were measured with the Pierce BCA Protein Assay Kit

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